Effektivisering av cyanobakteriers koldioxidfixering genom kartläggning av karboxysomens proteininteraktioner

Tidsperiod: 2020-01-01 till 2023-12-31

Projektledare: Cecilia Blikstad

Finansiär: Vetenskapsrådet

Bidragstyp: Bidrag för anställning eller stipendier

Budget: 4 400 000 SEK

To accelerate CO2 fixation, cyanobacteria encapsulate Rubisco and carbonic anhydrase within a protein organelle called the carboxysome. This provides Rubisco with a high concentration of CO2 and protects it from the costly process of photorespiration. The carboxysome is build out of thousands of individual proteins. However, molecular details of how all these protein-protein interactions robustly assemble and regulate the functional carboxysome are nearly nonexistent. Recent advances along with preliminary results suggest that intrinsically disordered proteins (IDPs) and regions (IDRs) are essential for carboxysome function. But this type of proteins are largely unstudied in bacteria. The objective of this proposal is to establish the role of IDPs and IDRs in carboxysome function, assembly and regulation. This will be accomplished by (I) studying the carbonic anhydrase, an essential component of the carboxysome shown to contain functional IDRs, and (II) using a proteome-wide approach, based on phage display, to map the carboxysome IDP and IDR interactome. Understanding assembly and regulation of this central part of CO2 fixation is expected to empower synthetic biology approaches aiming at enhancing photosynthetic efficiencies and growth rates in future energy and food crops such as cyanobacteria and plants. This project is intended to run full-time over four years, involving one to two persons, with the applicant as project leader.