Utveckling av Point-of-care Diagnostik för Multidrugsresistenta Escherichia coli i Kliniska Isolat från Nepal

Tidsperiod: 2019-01-01 till 2020-12-31

Projektledare: Suparna Sanyal

Medarbetare: Narayan Prasad Parajuli, Basudha Shrestha

Finansiär: Vetenskapsrådet

Bidragstyp: Stöd till forskningsmiljö

Budget: 800 000 SEK

The research question: Antibiotic resistance in bacteria is a global problem. Poverty and related socio-economic factors in Nepal have caused a huge increase in the multidrug resistant Escherichia coli (MDREC). Combating ´antibiotic resistance´ needs ´rational treatment´ - that requires early detection of the pathogen.What will be done:  We aim to develop a point-of-care diagnostics for the MDREC strains in clinical isolates from two hospitals (Manmohan Memorial and Kathmandu Model Hospital) in Nepal. The Nepalese team led by Dr. Basista P. Rijal and Dr. Basudha Shrestha will isolate DNA from the clinical isolates and ship to Sweden. The Swedish team led by Prof. Suparna Sanyal, will employ shotgun sequencing (at Sci-life Lab) and bioinformatics to identify species- and strain-specific biomarkers. DNA microchips will be developed for PCR or RPA based solid detection of MDREC.Mode of Collaboration: We will conduct two joint meetings each year, consisting of workshops and seminars. Experts from the antibiotic field will be invited. The researchers from Nepal will visit Uppsala 2-3 months / year. The Swedish researchers will spend 2 - 3 weeks in Nepal every year. The project will end with an international conference in Nepal.Impact: This project will aid in eradication of the antibiotic resistance problem. Junior researchers and students will receive technical and pedagogic training. If successful, this project will have a positive impact on the socio-economic status of Nepal.