Sensorisk nanocellulosa-baserad leveransplattform för sårläkande, bioaktiva molekyler (NanoCellSens)
Tidsperiod: 2019-01-01 till 2022-12-31
Projektledare: Natalia Ferraz
Bidragstyp: Bidrag för anställning eller stipendier
Budget: 3 600 000 SEK
The aim of this project is to lay the scientific foundation for the development of a new generation of nanocellulose-based therapeutic delivery platforms,with focus on the treatment of chronic wounds. Nanofibrillated cellulose (NFC) hydrogels that respond to the high level of reactive oxygen species (ROS) in chronic wounds by releasing bioactive molecules, and the potential of using these ROS-sensitive hydrogels as a platform for cell therapy, will be investigated. The specific tasks are: 1) synthesizing ROS-sensitive NFC hydrogels, investigating different types of ROS-responsive linkers, and optimizing the degree of crosslinking to obtain controlled release of model molecules, 2) evaluating the ROS-sensitive hydrogels when loaded with bioactive molecules (growth factors and cytokines), and investigating the release profile and the molecule´s bioactivity upon release, 3) evaluating the ROS-sensitive hydrogels as a platform for stem-cell therapy by determining cell adhesion, proliferation and biomolecule secretion profiles, both under normal cell culture conditions and in a ROS-rich environment, and 4) testing selected bioactive hydrogels in vivo for their ability to promote the resolution of hard-to-heal wounds in animal models. It is anticipated that the knowledge acquired will create the basis for developing a new generation of NFC hydrogels for biomedical applications and help to solve currently unmet needs in the treatment of chronic wounds.