Förvärvda strukturella kapsidfunktioner hos ikosahedrala dsRNA-virus: tre hypoteser

Tidsperiod: 2019-01-01 till 2022-12-31

Projektledare: Kenta Okamoto

Finansiär: Vetenskapsrådet

Bidragstyp: Bidrag för anställning eller stipendier

Budget: 4 800 000 SEK

Non-enveloped icosahedral dsRNA viruses that infect unicellular hosts are transmitted intracellularly. However, phylogenetically closely-related viruses found in multicellular hosts (metazoa) have acquired surface traits to facilitate an extracellular lifestyle. I will focus on a representative case of the icosahedral dsRNA virus; protozoan/fungal Totiviridae and metazoan totivirus-like viruses. Preliminary experiments indicate that the metazoan totivirus-like viruses have acquired three unique structural traits. Based on these results, I propose original hypotheses regarding these structural acquisitions in regards to their extracellular lifestyle. Function-deficient mutants of the totivirus-like virus will be constructed on the basis of these three acquired structures. These mutants will be evaluated for their effects on cell binding and infectivity, intra-particle genome synthesis, and particle stability to support my hypotheses. Finally, an atomic model of a fish totivirus-like virus will be determined to corroborate my hypothesis. The metazoan dsRNA viruses cause diverse problem for plant and animal ecology, and to human health. The acquired structural traits can be fundamental requirements for their transmission strategies, and therefore will be significant targets for developing vaccines and inhibitors.