Omfattande definiera cellulär identitet i början av neuronal differentiering

Tidsperiod: 2018-01-01 till 2021-12-31

Projektledare: Xingqi Chen

Finansiär: Vetenskapsrådet

Bidragstyp: Bidrag för anställning eller stipendier

Budget: 6 000 000 SEK

Uncovering the molecular mechanism driving cell heterogeneity would be great helpful in the clinical diagnosis, molecular basis of drug resistance in cancer and diseases therapy in the long term. Currently most of single cell studies only focus on one perspective of cellular behavior, either only DNA, RNA or protein expression. To fully understand the molecular mechanism determining the cell heterogeneity, it is essential to properly define cellular identity. However, technologies to simultaneously detect nucleus architecture, chromatin structure, protein expression and transcription are largely missing. The nervous system contains more than thousands sub cell types and is great system to understand the cell fate decision by identifying intermediately or terminally differentiated neural cells following neuronal stem cell differentiation. To understand the cell fate decision during neuronal differentiation, I would develop new technologies which could define the cell identity from completely angles, including nucleus architecture, chromatin structure, RNA transcription and protein expression at the same cell during early neutron differentiation with state-of-the-art imaging and multi-omics. Eventually the insight into the cellular identity and cell fate decision from this project could help us to understand the molecular mechanism driving cellular heterogeneity during neuron differentiation and make a toolkit for human clinical diagnosis of nervous-related disease and tumor.