Antimikrobiell resistens i miljön och betydelse för humanhälsa
Tidsperiod: 2017-01-01 till 2020-12-31
Projektledare: Josef Järhult
Bidragstyp: Bidrag för anställning eller stipendier
Budget: 6 000 000 SEK
Antimicrobial resistance is a rapidly growing health concern but environmental aspects are largely understudied. Here, a 2017-2020 multi-disciplinary project is proposed in which development and transfer of resistance in both influenza A virus and ESBL-producing Enterobacteriaceae (ESBLpEB) is investigated. The broad approach facilitates cost efficiency and a One Health-oriented view. Animal experiments using Mallards and chickens will be performed to investigate resistance development to the new anti-influenza drugs zanamivir, peramivir, and favipiravir in the natural host Mallards, persistence without drug pressure of such resistance, and the risk for transfer towards humans via reassortment and/or direct transmission via poultry. Screening, typing, and relatedness analysis of ESBLpEB and resistance genes will be performed in different compartments of the human-animal interface from high-income and low-income settings. Using broad screening studies and Mallard experiments, a special focus is put on birds because of their potential as reservoirs, spreaders, and sentinels of resistance. Methods employed include culturing of bacteria, PCR, Sanger sequencing, NGS, functional enzyme assays, egg- and cell culture. The project will contribute a basis for better influenza pandemic preparedness planning, more prudent use of antimicrobial drugs, risk assessment for food security, as well as surveillance and interventions including low-income countries.