Förändringar i elektronkonfigurationen och atomstrukturen hos biomolekyler inducerade av röntgenlaserpulser
Tidsperiod: 2014-01-01 till 2017-12-31
Projektledare: Carl Caleman
Budget: 3 700 000 SEK
Biological imaging using Free-Electron Laser (FEL) pulses has in the last two years manifested its great potential. In summary we have demonstrated: i) That it is possible to get high quality atomic resolution structural information from proteins in nano-sized crystals, using the so called serial femtosecond crystallography (SFX) technique ii) That we can retrieve the unknown atomic structure of a protein using SFX. Science magazine listed this technique as one of the major scientific breakthroughs 2012, and there is no doubt SFX could become an important complement to conventional X-ray crystallography.Despite the great progress in the field, the there are many issues that have to be dealt with. One of the major problems with the methodology is the radiation damage induced by the intense X-ray pulses. The fast electronic rearrangements caused by the ionization, as well as the displacement of the atomic positions, have a direct effect on the diffracted X-ray signal from the sample. This project will focus on that specific problem. I aim to undertake research with the following specific aims: i) perform experiments to investigate ultrafast dynamics in FEL exposed nanocrystals and single bio-molecules. This will improve existing reconstruction algorthims and increase the gerenality of the technique, ii) develop theoretical models for damage dynamics in the high field regime, that can be used in understanding experimental data and guide future experiments.