Kan HIV-infektion förhindras - karakterisering av tidig värdrespons i SIV-infekterade makaker med RNA-sekvensering

Tidsperiod: 2013-07-01 till 2016-06-30

Projektledare: Fredrik Barrenäs

Finansiär: Vetenskapsrådet

Bidragstyp: Projektbidrag

Budget: 3 150 000 SEK

The HIV/AIDS pandemic is still one of the most serious threats to human health, causing 1.7 million deaths in 2011 alone. Efforts to produce a vaccine to prevent HIV infection, which is needed to limit the disease globally, have been largely unsuccessful to date. The immune system plays a complex role in AIDS development. While anti-viral mechanisms appear to limit HIV infection immediately following infection, over time a protracted inflammation develops during which the immune cells are depleted and the virus proliferates. However, how the immune system prohibits HIV proliferation is not known. SIV infection in rhesus macaques is similar to human HIV infection; they exhibit both the early suppression of SIV proliferation and develop an AIDS-like syndrome. They are therefore the most accurate animal model of HIV infection. The aim of this proposed project is to characterize host response during the early phase and ensuing chronic inflammation following SIV infection of Indian-origin rhesus macaques. The project is primarily based on bioinformatic analysis of transcriptomic data, obtained by next generation sequencing. The University of Washington, as host institute, will provide sophisticated RNA sequencing data from immune system tissues, such as PBMCs, lymph nodes, and mucosal immune compartments. Time series data from SIV infection have been obtained by sacrificing animals at set time points following SIV inoculation.