Enkelcellsgenomik av marina arkéer

Tidsperiod: 2013-01-01 till 2015-12-31

Projektledare: Erik Pelve

Finansiär: Vetenskapsrådet

Bidragstyp: Projektbidrag

Budget: 3 150 000 SEK

Archaea are present in all ecosystems as important facilitators of global cycling of nutrients. In the deep ocean they are as common as bacteria which make the archaea one of the most numerous groups of organisms on Earth. They constitute a unique linage of living cells, as different from bacteria as bacteria are from eukaryotes. Despite their significance for vital ecosystems, important aspects of archaeal ecology remain unknown.The goal of this project is to sequence the genomes of single archaeal cells in marine systems. The cells are sampled at the Hawaii ALOHA marine station and the Chile oxygen minimum zone. Target single cells will be isolated by flow cytometric cell sorting, and the genomic content sequenced using modern high throughput sequencing technologies.The sequenced genome of the marine archaea will be analyzed from metabolic, phylogenetic, evolutionary and ecological perspective. Metagenomic datasets from different depths and seasons have been collected by the DeLong lab and will be used to explore the population dynamics of the sequenced archaea. Specifically, this project will test the hypothesis of heterotrophy in deep ocean living archaea, involvement in ammonia oxidization and evolutionary and ecological dynamics.The project takes a cellular perspective to understand the oceanic ecosystems. We utilize cutting edge application of modern sequencing technology to determine the ecological function of one of the most common organism in the world.