En modell av oligotrofa bakterier med effektiviserat genom för mikrobiologi
Tidsperiod: 2013-01-01 till 2016-12-31
Budget: 4 000 000 SEK
There is a current gap between molecular and ecological microbiology as the abundant and ecosystem-relevant microbes are scarcely characterized in regards to their metabolism, cell-division, expression, DNA exchange and repair. In this project strains belonging to the most widespread and abundant aquatic bacteria, called Pelagibacteraceae, will be established as models to test hypotheses derived from computational predictions of genomic data. Simple growth experiments using cultured isolates will be used to verify metabolic reconstructions. Oligotrophic bacteria, such as Pelagibacteraceae play a central role in the cycling of dissolved organic matter and determining which carbon compounds are used by Pelagibacteraceae will serve as a resource for aquatic chemists and biogeochemists working to define the carbon cycle in greater mechanistic detail and at large geographical scales. Comparative genomics and experiments will also reveal the traits underlying their marine-freshwater transition. Finally, we will also investigate if high rates of recombination predicted for Pelagibacteraceae are selectively advantageous in aquatic environments. Here, evolution in controlled experimental settings using antibiotics as a selective pressure will help towards understanding homologous recombination and adaptation in oligotrophic bacteria with streamlined genomes. This will also provide the means to better predict the spread of antibiotic resistance genes in natural environments.