Eosinofila granulocytens sekretoriska proteiner vid hälsa och sjukdom

Tidsperiod: 2013-01-01 till 2015-12-31

Projektledare: Per Venge

Finansiär: Vetenskapsrådet

Bidragstyp: Projektbidrag

Budget: 1 800 000 SEK

The overall aim of this project is the understanding of the role of the human eosinophil granulocyte in health and disease. Our strategy is to approach the question from three angels. 1: The identification, purification and characterization of novel secretory proteins. 2:The study of structure-function relationships of these and previously identified proteins. 3: Define single nucleotide polymorphisms in the genes of key proteins that may impact on the activity and/or the production of the proteins and investigate the relationship of such polymorphisms to human disease. Specifically our aims are accomplished by: 1. The purification and characterization of novel proteins contained in the secretory granules 2. The purification of ECP and EPX/EDN from genetically defined native sources 3. Detailed study of the structure-function relationships of ECP, EPX/EDN and EPO 4. Study of the genetic mechanisms governing the production of ECP and EPX/EDN 5. Genetic studies of functional gene polymorphisms of the ECP, EPX/EDN and EPO genes in cohorts of patients with eosinophil involving disease such as asthma/allergy, COPD, smokers, parasitic disease, inflammatory bowel disease and certain tumors 6. The study of eosinophil granule protein secretions 7. Proteomics of eosinophils and their secretory products in eosinophils of healthy and diseased subjects. 8. Development of sensitive immunoassays for the detection of the novel proteins in human biological fluids.