Mångsidig och hållbar enzymkatalyserad kemi

Tidsperiod: 2012-01-01 till 2014-12-31

Finansiär: Vetenskapsrådet

Bidragstyp: Projektbidrag

Budget: 2 150 000 SEK

The possibility to harness the extraordinary catalytic efficiencies and reaction selectivities of enzymes has long appealed to synthetic chemists. The full biodegradability and non-toxicity of proteins also add the possibility of sustainable production of useful chemicals. The concept of biocatalysis describes such synthesis regimes where enzymes are used as reaction catalysts.This present project extends on the current development of new biocatalysts in that sequential enzyme-catalyzed transformations of organic molecules are aimed for. The enzymes employed have been engineered for desired functions.The epoxide functional group is a starting point for synthesis of derivative compounds. Epoxides are relevant as precursors in chiral chemistry where the reactant epoxide and the ring-opened product contain one or more chiral centers. Epoxides are readily hydrolyzed by epoxide hydrolase to yield (chiral) vicinal diols. Such diols can be oxidized at the primary or secondary alcohol carbons by alcohol dehydrogenases of distinct regiospecificities. The resulting aldehydes and ketones can be further derivatized. Chemistry allowing for carbon-carbon bond formation is of high synthetic value and a commonly used approach is through aldol condensation of carbonyl compounds. Aldolase enzymes possess the ability to condense either a ketone with an aldehyde, or two aldehydes into aldols. Alternatively, ketones can be transformed into chiral amines by aminotransferases