Kopplad veckning och bindning hos proteiner
Tidsperiod: 2012-01-01 till 2015-12-31
Projektledare: Stefan Knight
Budget: 4 300 000 SEK
Life processes depend critically on interactions between macromolecules. Many of these interactions involve coupled folding and binding, which lies at the heart of many biologically, medically, and technologically important processes and phenomena. The assembly of adhesive organelles via the chaperone/usher pathway provides an elegant example of how protein folding by Nature ìs design may be coupled to binding in order to gain control over a complex assembly and secretion process. Spider silks, assembled in a complex process that links folding to polymerization, have extraordinary mechanical properties that make them attractive for development of novel materials. BRICHOS domains are used by mammalian cells to manage the problems inherent in aggregation-prone peptides that must be partitioned away from amyloidogenic pathways into biologically meaningful ones. These examples of coupled folding and binding will be studied using a powerful combination of X-ray crystallography, fibre diffraction, small angle scattering, molecular biology, biophysical, biochemical, and computational techniques. The knowledge generated from these studies will shed light on fundamental questions important for many biological processes. Such knowledge paves the way for development of novel applications in e.g. medicine and biotechnology.