Design av cyanobakterier för vätgasproduktion från solenergi och vatten

Time period: 2012-01-01 to 2014-12-31

Funder: Swedish Energy Agency

Type of award: Project grant

Total fundning: 3 315 000 SEK

This is a new research project aiming at the design and construction of cyanobacterial cells for the production of renewable H2 using a synthetic biology approach. It is based on own competence on cyanobacterial hydrogenases and H2 metabolism in combination with recent advances in Synthetic biology, specifically our own progress on developing Synthetic biology tools for cyanobacterial biotechnology. The aim of the present project is to take cyanobacterial biotechnology into a higher level by using Synthetic biology as an advanced molecular tool not only to introduce novel genetic information into the cells but also to do it in a standardized engineering manner. Specifically, more O2 insensitive non-cyanobacterial prokaryotic NiFe-hydrogenaes as well as more efficient eukaryotic FeFe-hydrogenases will be introduced into selected cyanobacterial cells (Synechocystis and Nostoc). All introduced genetic information will be artificially synthesized making it possible to e.g. codon optimize for selected hosts and to use DNA-sequences never evolved in nature. The fate of the introduced foreign genetic information will be followed in the cyanobacterial cells using a combination of transcriptomic and proteomic technologies. Examples of issues to address: Transcription and translation of the introduced DNA into stable transcripts and functional/non-functional proteins. Integration into general metabolism and specifically different electron transfer pathways. H2 production capacities.