Drug delIvery system for enhanced targeting of glioblastoma multiforme (GBM)

Time period: 2020-07-01 to 2023-06-30

Project leader: Bo Nilsson

Funder: Vinnova

Type of award: Project grant

Total fundning: 2 000 000 SEK

Purpose and goal: The purpose of Uppsala Universitet (UU) in the RELIEF project is to produce liposomes that can search for brain tumors called glioblastoma multiforme, via blood vessels. Previous experiments with similar technology have shown that the liposomes disappear from the bloodstream because our humoral innate immune system considers the liposomes as foreign particles to be destroyed just like foreign microorganisms. The goal is to coat the liposomes with protective molecules that can also be used to hook targeted molecules. Two newly developed linings will be used. Expected results and effects: The project will enable us to take our newly developed liposome coatings from the laboratory level to GMP-production. We expect a double impact of this on our part of the RELIEF project. First, the success of our new coating of liposomes will lead to a breakthrough in an area where few innovative improvements have been presented since the introduction of PEG-coated liposomes in the 90s. Secondly, we expect that revenues from licensing fees will support the project´s continued development within UU for alternative applications on materials and cells. Approach and implementation: UU will primarily be active in RELIEF at the beginning of the project. Two alternative liposome coatings will be further developed for RELIEF eg low molecular weight heparin which binds to the lipids. Conjugation of targeting peptides will also be performed. These liposomes as well as a library of different liposomes of varying size, composition and coated with different coatings of varying density will be tested in vitro for its blood-compatible properties, i.e., their ability to prevent activation of the humoral natural immune system in blood.