Complete DNA from a 35,000 years old woman sequenced

Skull of Peştera Muierii 1, a woman who lived in today’s Romania 35,000 years ago..

Skull of Peştera Muierii 1, a woman who lived in today’s Romania 35,000 years ago..

For the first time, researchers have successfully sequenced the entire genome from the skull of Peştera Muierii 1, a woman who lived in today’s Romania 35,000 years ago. Her high genetic diversity shows that the out of Africa migration was not the great bottleneck in human development but rather this occurred during and after the most recent Ice Age. This is the finding of a new study led by Mattias Jakobsson at Uppsala University.


“She is a bit more like modern-day Europeans than the individuals in Europe 5,000 years earlier, but the difference is much less than we had thought. We can see that she is not a direct ancestor of modern Europeans, but she is a predecessor of the hunter-gathers that lived in Europe until the end of the last Ice Age,” says Mattias Jakobsson, professor at the Department of Organismal Biology at Uppsala University and the head of the study.

Similarities with modern humans

Very few complete genomes older than 30,000 years have been sequenced. Now that the research team can read the entire genome from Peştera Muierii 1 (see the fact box below), they can see similarities with modern humans in Europe while also seeing that she is not a direct ancestor. In previous studies, other researchers observed that the shape of her cranium has similarities with both modern humans and Neanderthals. For this reason, they assumed that she had a greater fraction of Neanderthal ancestry than other contemporaries, making her stand out from the norm. But the genetic analysis in the current study shows that she has the same low level of Neanderthal DNA as most other individuals living in her time. Compared with the remains from some individuals who lived 5,000 years earlier, such as Peştera Oase 1, she had only half as much Neanderthal ancestry.

Greatest loss of genetic diversity during the last Ice Age

The spread of modern humans out of Africa about 80,000 years ago is an important period in human history and is often described as a genetic bottleneck. Populations moved out of Africa and into Asia and Europe. The effects of these migrations can be seen even today. Genetic diversity is lower in populations outside of Africa than in African. That Peştera Muierii 1 has high genetic diversity implies that the greatest loss of genetic diversity occurred during the last Ice Age (which ended about 10,000 years ago) instead of during the out of Africa migration.

“This is exciting since it teaches us more about the early population history of Europe. Peştera Muierii 1 has much more genetic diversity than expected for Europe at this time. This shows that genetic variation outside of Africa was considerable until the last Ice Age, and that the Ice Age caused the decrease in diversity in humans outside of Africa.”

The researchers were also able to follow the genetic variation in Europe over the last 35,000 years and see a clear decrease in variations during the last Ice Age. The reduced genetic diversity has previously been linked to pathogenic variants in genomes being more common among populations outside of Africa, but this is in dispute.

Access to advanced medical genomics

“Access to advanced medical genomics has allowed us to study these ancient remains and even be able to look for genetic diseases. To our surprise, we did not find any differences during the last 35,000 years, even though some individuals alive during the Ice Age had low genetic diversity.

Now we have accessed everything possible from these remains. Peştera Muierii 1 is important from a cultural history perspective and will certainly remain interesting for researchers within other areas, but from a genetic perspective, all the data is now available.”

Elin Bäckström

Fact Peştera Muierii


Peştera Muierii 1 is the name given to one of the three individuals whose remains were found in a cave of the same name. Peştera Muierii (roughly translates to women’s cave) is the name of a cave system in Baia de Fier in southern Romania. It is best known for the remains of cave bears and for the 1950s discovery of skulls and other skeletal parts from three females that lived about 35,000 to 40,000 years ago.

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